mouse antipig ba4d5  (Bio-Rad)

Journal: Microbiology Spectrum

Article Title: African swine fever virus genes vectored by simian adenoviruses do not protect against virulent genotype II virus challenge

doi: 10.1128/spectrum.02328-25

Figure Lengend Snippet: Antigen-specific CD8 T-cell responses. Animals were immunized with eight vectors (red), GFP (gray), or nine vectors (blue) and boosted 28 days later. PBMCs purified on the indicated days were stimulated with peptide pools corresponding to the indicated gene products or the ubiquitinylated polyprotein (SW). The proportion of CD3 + CD8α + CD4 cells expressing IFNγ ( A–D ) or IFNγ and CD107a ( E–H ) 2 weeks post-prime ( A, D ), pre-boost ( B, E ), 7 days post-boost ( C, F ), and pre-challenge ( D, H ) was determined by flow cytometry. Each data point indicates a single animal, and bars show the mean of each group. Green fluorescent protein (GFP); interferon gamma (IFNγ); peripheral blood mononuclear cell (PBMC). * P ≤ 0.05, ** ≤ 0.01 .

Article Snippet: CD107a , 4E9/11 , Mouse IgG1 , Alexa Fluor 647 , 1:100 , Bio-Rad Laboratories , MCA2315A647.

Techniques: Purification, Expressing, Flow Cytometry

Journal: Microbiology Spectrum

Article Title: African swine fever virus genes vectored by simian adenoviruses do not protect against virulent genotype II virus challenge

doi: 10.1128/spectrum.02328-25

Figure Lengend Snippet: Virus-specific CD8 T-cell responses. Animals were immunized with eight vectors (red), GFP (gray), or nine vectors (blue). Cells were purified from blood collected on the indicated days and stimulated with mock ( A, B ), OUR T1988/1 ( C, D ), or Georgia 2007/1 ( E, F ) inocula overnight and then treated with brefeldin A and anti-CD107a for 4 h. Populations of CD3 + CD8α + CD4 cells were then identified by flow cytometry, and the proportion of them expressing either IFNγ ( A, C, E ), IFNγ and CD107a ( B, D, F ) was determined. Each data point indicates a single animal, and bars show the mean of each group. Green fluorescent protein (GFP); interferon gamma (IFNγ). * P ≤ 0.05, ** ≤ 0.01 .

Article Snippet: CD107a , 4E9/11 , Mouse IgG1 , Alexa Fluor 647 , 1:100 , Bio-Rad Laboratories , MCA2315A647.

Techniques: Virus, Purification, Flow Cytometry, Expressing