2a10 11  (Bio-Rad)

Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Testicular immunity and orchitis under HS conditions. A and B GO (A) and KEGG (B) enrichments based on Gene Set Enrichment analysis. C Protein-protein interaction network. D Representative photos of collagen fibers stained by Masson in the testes. E and F Levels of TNF-α (E) and IL-1β (F) in plasma isolated from the spermatic vein. G Representative flow cytometry quantifications of macrophages in the testes. H and I Percentages of CD68 + CD163 + and CD68 + cells among CD45 + cells in the testes. * P < 0.05, ** P < 0.01

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Staining, Clinical Proteomics, Isolation, Flow Cytometry

Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Changes in the TM profile and complement pathway under HS conditions. A tSNE graph of identified cells. B Percentages of cells in each group. C KEGG enrichment of the DEGs from TMs. D Expression levels of C1QA , C1QB , and C1QC in testicular cells as assessed by scRNA-seq. E tSNE graph of TM subclusters in each group. F Percentages of subclusters in each group. G KEGG enrichment of marker genes in Subcluster 5. H Expression levels of C1QA , C1QB , and C1QC in TM subclusters as assessed by scRNA-seq. I KEGG enrichment of DEGs from TM Subcluster 5. J Addmodulescore of subclusters. K tSNE graph of C1QA , C1QB , C1QC , and CD163 as assessed by scRNA-seq

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Expressing, Marker

Journal: Journal of Animal Science and Biotechnology

Article Title: Heat stress induced testicular impairment is related to orchitis and complement activation in Rongchang boars

doi: 10.1186/s40104-025-01296-5

Figure Lengend Snippet: Testicular immunity and orchitis under HS conditions. A and B GO (A) and KEGG (B) enrichments based on Gene Set Enrichment analysis. C Protein-protein interaction network. D Representative photos of collagen fibers stained by Masson in the testes. E and F Levels of TNF-α (E) and IL-1β (F) in plasma isolated from the spermatic vein. G Representative flow cytometry quantifications of macrophages in the testes. H and I Percentages of CD68 + CD163 + and CD68 + cells among CD45 + cells in the testes. * P < 0.05, ** P < 0.01

Article Snippet: The remaining cells were resuspended in 100 μL Stain Buffer (BD, Franklin Lakes, New Jersey, USA) containing mouse anti-pig CD45: FITC (Bio-Rad, Hercules, California, USA), mouse anti-pig CD163: RPE (Bio-Rad, Hercules, California, USA), and BV421 rat anti-CD11b (BD, Franklin Lakes, New Jersey, USA) at 4 °C for 30 min. Each suspension was centrifuged at 350 × g for 5 min at 4 °C to remove the supernatant.

Techniques: Staining, Clinical Proteomics, Isolation, Flow Cytometry